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Autoclave

Must have biohazard training to have media autoclaved.
Turn it into Barbara in the morning, all done by afternoon.
No autoclaving of nasty chemicals or volatile materials.

 

You MUST be biohazard trained to have media autoclaved and/or working with cultures.

NO AUTOCLAVING of nasty materials of the volatile kind.
No solvents, minerals acids, volatile poisons, etc.

There is one autoclave at Hopkins:

- Turn your material into Barbara in the morning and in the afternoon you pick it up. Wow! Simple!
- No hassles. Large quantities can be done at once.

There are times when you absolutely need to have some media done at 3am or on a weekend [really?]. Sorry, not going to happen. Plan ahead.

Why such a hassle? I used autoclaves at my last place. Why can't I use them here?

AN AUTOCLAVE IS POTENTIALLY A LETHAL OR MAIMING INSTRUMENT.

High pressure, high temperature water and steam exposure to humans can be disastrous. They only way to insure their safe operation is to have people who are very knowledgeable in their operation.

NO media will be autoclaved for any individual who is not biohazard trained.

Helpful Hints:

  1. PACK LOOSE!
    If steam does not contact the surface the bacteria are not killed.

  2. Insure that each package is covered/protected with a plastic autoclave bag, aluminum foil, or similar arrangement. [BUT NOT AIR TIGHT!]
    IMPORTANT: Check old foil for pinholes. One hole = non-sterile!

  3. Using the autoclave tape on the cart in front of the autoclaves, attach a piece to each package.
    Leave a name and # if you want to be called after agar media is done (so you can pour it before it cools).

  4. Label the autoclave tape with either DRY or FLUID and your lab name.

  5. If this is a rush, check with the Barbara. Do not assume that your request can be honored.

  6. After autoclaving, your package will be on the back counter near the ice machine. Wait until your package is cool enough to transport to your lab or research area. Liquids can "EXPLODE" out of containers if they are just out of the autoclave and are jarred or shaken.

melted carboy from autoclave

 

BE SURE THE MATERIAL YOU ARE ASKING TO BE AUTOCLAVED
CAN IN FACT BE AUTOCLAVED. IN OTHER WORDS,
IT CAN HANDLE THE HEAT AND PRESSURE.

 

Biohazardous Waste:

  1. DO NOT OVER FILL! Cannot exceed 14 lbs either.

  2. Must be in a red Hopkins biohazard waste pail.

  3. Label with contents type and lab (rDNA, cDNA, bacterial, BL-2, etc)(Block, Palumbi, etc.)

PROBLEMS?

Check with Barbara, x56224 and report to Judy Thompson, x56249